IPERION HSIntegrating Platforms for the European Research Infrastructure ON Heritage Science

Size-Exclusion Chromatography (SEC)

SEC is a type of liquid chromatography, which enables the separation of molecules according to their size. The main characteristic of stationary phases are different sized pores. Smaller molecules enter these pores and therefore they move along the column slowly in comparison to larger molecules. Some molecules are too large to enter the pores and they are excluded and consequently have no retention.

Fields of application

  • Cultural heritage

    manuscript, painting, papyrus, textile


  • organic

    animal parts, binding media, glues, wood, paper, textiles, varnishes, dyes, inks


Size-Exclusion Chromatograph

Size-Exclusion Chromatograph with refractive index detector (RefractoMax520), UV detector (RS Variable Wavelength Detector), viscometer and multi angle light scattering detectors; Termo Scientific Dionex UltiMate 3000 UHPLC.