IPERION HSIntegrating Platforms for the European Research Infrastructure ON Heritage Science
Mass Spectrometry applied to Cultural Heritage

The Heritage Science Molecular Omics Platform organizes in the frame of the EU_FT-ICR_MS Horizon 2020 infrastructure from Thursday, November 24th to Saturday, November 26th in Lille (France) a Mass Spectrometry Short course for Cultural Heritage focused on the identification of natural products preserved over the centuries which are very often polymeric. The short course will begin with brief theoretical courses covering an overview of Mass Spectrometry applied to Cultural Heritage, the principle of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS), the concept of fine isotopic structure as well as the basis of proteomics, glycomics and lipidomics.

The practical sections of the training comprise examples from these various topics. Firstly, a demonstration of sample preparation will be held: (i) collagen extraction and digestion with minimal induction of posttranslational modifications, quantification of deamidation; (ii) digestion of model gums, watercolour or gouache modern paint and (iii) and depolymerization of alkyd artist paint. Subsequently, the prepared samples will be analyzed with high-resolution MALDI FT-ICR and nanoLC Orbitrap results. The obtained spectra will be further compared to their corresponding ones acquired through lower resolution instruments such as MALDI TOF MS. Finally, for proteomics, a comparison related to data treatment will show different outcomes from the same spectra depending on the database sources, with publicly available and in-house established databases.

Travel, accommodation and meals for European participants are taken into charge by the EU (EU_FT-ICR_MS network, Grant Agreement 73107).

Deadline: before November 14, 2022