IPERION HSIntegrating Platforms for the European Research Infrastructure ON Heritage Science
Technique: Enzyme-Linked ImmunoSorbent Assays (ELISA)

Microplate Photometer

Multiskan™ FC 96-well Microplate Photometer has a 340–850 nm wavelength range, enabling a wide variety of applications from enzyme catalised colourimetric product measurement in ELISA assay to to Lowry assays (96-well and 384-well plate formats are allowed). It also provides linear shaking in temperature ranges of up to 50°C.

Potential Results

The tool can be used for Enzyme-Linked Immuno Sorbent Assays (ELISA) for the characterisation and measurement of organic materials in cultural heritage samples. For example, the technique provides a positive identification of specific proteins in the removed sample (e.g. there is chicken ovalbumin in the removed paint layer; there is bovine collagen in the sample removed from a glue layer).