UV-Vis fluorescence time-decay

The radiative deactivation pathway of a molecular electronic excited state is characterized by a fast kinetic, ranging from picoseconds to few seconds, that leads to the ground state recovering. Using a pulsed excitation source, it is possible to measure the kinetic parameter of the fluorescence decay which is called lifetime. In the occurrence of fluorophores with similar emission spectra, the fluorescence decay lifetimes can be exploited for distinguished fluorescing materials.